antitomm20 (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology antitomm20
Antitomm20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2725 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antitomm20/Tom20+Antibody/pm41016591-59-19-30
Average 96 stars, based on 2725 article reviews

antitomm20 - by Bioz Stars, 2026-10

96/100 stars

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Article Title: Identification of a mitochondrial targeting sequence in cathepsin D and its localization in mitochondria.
Article Snippet: Proteins separated with SDS-PAGE were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). .. Membranes were blocked with 5% skimmilk in Tris-buffered saline with 0.05% Tween 20 for 1 h. Membranes were incubated with primary antibodies at 4 C overnight, and subsequently with secondary antibodies for 1 h. Antibodies used were as follows: anti-CTSD mouse monoclonal (1:1000, CTD-19, NOVUS), anti-CTSD goat polyclonal (1:250, sc-6486, Santa Cruz Biotechnology), antiTOMM20 (1:250, sc-17764, Santa Cruz Biotechnology), antiATP5F1B (1:400, PAB28448, Abnova), anti-SEC22B (1:200, sc101267, Santa Cruz Biotechnology), anti-Golgin97 (1:200, sc59820, Santa Cruz Biotechnology), anti-beta-actin (1:5000, A5316, Sigma-Aldrich), and anti-GFP (sc-9996, Santa Cruz Biotechnology), anti-rabbit IgG-HRP (1:5000, sc-2004, Santa Cruz Biotechnology), anti-mouse IgG-HRP (1:5000, sc-2005, Santa Cruz Biotechnology), and anti-goat IgG-HRP (1:5000, sc-2020, Santa Cruz Biotechnology) 2.11. .. Analysis of CTSD localization in fixed cells HeLa cells transfectedwith pEGFP-CTSDwere subjected to none, one, pairs, or all of the following procedures for immunofluorescence (IF), 24 h after transfection. i) Fixation with 4% paraformaldehyde for 15 min. ii) Permeabilization with 0.1% Triton X100 for 5 min iii) Subsequent procedures for antigen-antibody reaction: washing 3x with phosphate-buffered saline (PBS) at room temperature, blocking with 3% bovine serum albumin (BSA) in PBS for 1 h, incubating with primary antibody, anti-TOMM20 (1:100, sc17764, Santa Cruz Biotechnology) or anti-cytochrome c (1:100, 6H2.B4, Imgenex) in 3% BSA in PBS for 2 h at room temperature, washing 3x with PBS, incubating with secondary antibody, Alexa Fluor 546 (1:200, A-11003, Thermo Fisher Scientific) in 3% BSA in PBS for 1 h at room temperature, and washing 3x with PBS.

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Article Title: AMPK activation mitigates α-synuclein pathology and dopaminergic degeneration in cellular and mouse models of Parkinson's disease.
Article Snippet: Parkinson’s disease (PD) is characterized by oxidative stress, mitochondrial dysfunction, and pathological accumulation of p-α-Synuclein (p-α-Syn).. AMP-activated protein kinase (AMPK) has emerged as a regulator of cellular energy homeostasis, yet its role in PD pathology remains unclear.. Here, we examined the effects of AMPK activation in SH-SY5Y neuroblastoma cells and in an MPTP-induced PD mouse model.

Article Title: Palmitoylation-mediated NLRP3 inflammasome activation in teleosts highlights evolutionary divergence in immune regulation.
Article Snippet: Antibodies and compounds used in this study included: antiTOMM20 (1:200, #sc-17764, Santa Cruz Biotechnology, USA), anti-Calnexin (1:100, #sc-23954, Santa Cruz Biotechnology, USA), anti-γ-tubulin (1:500, #T6557, Sigma Aldrich, USA), anti-TGN46 (1:100, #sc-166594, Santa Cruz Biotechnology, USA), anti-ninein (1:100, #sc-376420, Santa Cruz Biotechnology, USA), goat anti-mouse IgG2b Crossadsorbed secondary antibody, Alexa FluorTM 555 (1:500, #A21147, Thermo Fisher Scientific, USA), goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa FluorTM 488 (1:500, #A-11008, Thermo Fisher Scientific, USA), goat antirabbit IgG, horseradish-peroxidase (HRP) conjugated secondary antibody (1:3000, #M21002, Abmart, China), goat anti-mouse IgG, HRP conjugated secondary antibody (1:3000, #M21001, Abmart, China), mouse anti-HA (1:3000, #M20003, Abmart, China), rabbit anti-HA (1:3000, #TT0050, Abmart, China), rabbit anti-FLAG tag (1:3000, #TT0053, Abmart, China), mouse anti-FLAG tag (1:3000, #M20008, Abmart, China), mouse anti-Myc (1:3000, #M20002, Abmart, China), anti-β-tubulin (1:2000, #M20005, Abmart, China), anti-GAPDH (1:2000, M20006, Abmart, China), anti-FLAG® M2 affinity gel (#A2220, Sigma-Aldrich, USA), monoclonal anti-HA-agarose (#A2095, Sigma-Aldrich).

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